diff --git a/libavformat/audiointerleave.c b/libavformat/audiointerleave.c
index 6d4954befe..dea5d99821 100644
--- a/libavformat/audiointerleave.c
+++ b/libavformat/audiointerleave.c
@@ -81,15 +81,19 @@ static int interleave_new_audio_packet(AVFormatContext *s, AVPacket *pkt,
     AVStream *st = s->streams[stream_index];
     AudioInterleaveContext *aic = st->priv_data;
     int ret;
-    int size = FFMIN(av_fifo_size(aic->fifo), *aic->samples * aic->sample_size);
+    int frame_size = *aic->samples * aic->sample_size;
+    int size = FFMIN(av_fifo_size(aic->fifo), frame_size);
     if (!size || (!flush && size == av_fifo_size(aic->fifo)))
         return 0;
 
-    ret = av_new_packet(pkt, size);
+    ret = av_new_packet(pkt, frame_size);
     if (ret < 0)
         return ret;
     av_fifo_generic_read(aic->fifo, pkt->data, size, NULL);
 
+    if (size < pkt->size)
+        memset(pkt->data + size, 0, pkt->size - size);
+
     pkt->dts = pkt->pts = aic->dts;
     pkt->duration = av_rescale_q(*aic->samples, st->time_base, aic->time_base);
     pkt->stream_index = stream_index;
@@ -99,7 +103,7 @@ static int interleave_new_audio_packet(AVFormatContext *s, AVPacket *pkt,
     if (!*aic->samples)
         aic->samples = aic->samples_per_frame;
 
-    return size;
+    return pkt->size;
 }
 
 int ff_audio_rechunk_interleave(AVFormatContext *s, AVPacket *out, AVPacket *pkt, int flush,
diff --git a/tests/ref/lavf/mxf b/tests/ref/lavf/mxf
index 30a7846143..9f735e9bd2 100644
--- a/tests/ref/lavf/mxf
+++ b/tests/ref/lavf/mxf
@@ -1,9 +1,9 @@
 93367bc02b8997b84224e7eb359cae8a *./tests/data/lavf/lavf.mxf
 526393 ./tests/data/lavf/lavf.mxf
 ./tests/data/lavf/lavf.mxf CRC=0x8dddfaab
-0087ea968d20cc9192456fcd4c00019e *./tests/data/lavf/lavf.mxf
+0e6d7a2a2c99a2a168114698be4c645f *./tests/data/lavf/lavf.mxf
 561721 ./tests/data/lavf/lavf.mxf
-./tests/data/lavf/lavf.mxf CRC=0xf21b1b48
+./tests/data/lavf/lavf.mxf CRC=0x96ff1b48
 7754550ff6fe5895555610f586a15fc5 *./tests/data/lavf/lavf.mxf
 526393 ./tests/data/lavf/lavf.mxf
 ./tests/data/lavf/lavf.mxf CRC=0x8dddfaab