diff --git a/libavformat/utils.c b/libavformat/utils.c
index c19238ea74..c4e57f66d8 100644
--- a/libavformat/utils.c
+++ b/libavformat/utils.c
@@ -2016,6 +2016,7 @@ static void av_estimate_timings_from_pts(AVFormatContext *ic, int64_t old_offset
         st= ic->streams[i];
         st->cur_dts= st->first_dts;
         st->last_IP_pts = AV_NOPTS_VALUE;
+        st->reference_dts = AV_NOPTS_VALUE;
     }
 }