diff --git a/tests/fate/prores.mak b/tests/fate/prores.mak
index 56568b2d42..1a3380a237 100644
--- a/tests/fate/prores.mak
+++ b/tests/fate/prores.mak
@@ -3,6 +3,7 @@ FATE_PRORES = fate-prores-422                                           \
               fate-prores-422_lt                                        \
               fate-prores-422_proxy                                     \
               fate-prores-alpha                                         \
+              fate-prores-transparency                                  \
 
 FATE_SAMPLES_AVCONV-$(call DEMDEC, MOV, PRORES) += $(FATE_PRORES)
 fate-prores: $(FATE_PRORES)
@@ -12,3 +13,4 @@ fate-prores-422_hq:    CMD = framecrc -flags +bitexact -i $(SAMPLES)/prores/Sequ
 fate-prores-422_lt:    CMD = framecrc -flags +bitexact -i $(SAMPLES)/prores/Sequence_1-Apple_ProRes_422_LT.mov -pix_fmt yuv422p10le
 fate-prores-422_proxy: CMD = framecrc -flags +bitexact -i $(SAMPLES)/prores/Sequence_1-Apple_ProRes_422_Proxy.mov -pix_fmt yuv422p10le
 fate-prores-alpha:     CMD = framecrc -flags +bitexact -i $(SAMPLES)/prores/Sequence_1-Apple_ProRes_with_Alpha.mov -pix_fmt yuva444p10le
+fate-prores-transparency: CMD = framecrc -flags +bitexact -i $(SAMPLES)/prores/prores4444_with_transparency.mov -pix_fmt yuva444p10le
diff --git a/tests/ref/fate/prores-transparency b/tests/ref/fate/prores-transparency
new file mode 100644
index 0000000000..3842970cd2
--- /dev/null
+++ b/tests/ref/fate/prores-transparency
@@ -0,0 +1,5 @@
+#tb 0: 1/25
+#tb 1: 1/48000
+0,          0,          0,        1, 16588800, 0x87f98f3c
+1,          0,          0,     1024,     4096, 0x00000000
+1,       1024,       1024,      896,     3584, 0x00000000